External factors
| External factors: |
Wogonin |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//RT-qPCR//Western blot//ROS Assay//Immunofluorescence |
| Description: |
Compared with control cells, more wogonin-treated cells were positively stained in theβ-galactosidase staining assay. Consistent with this, RT-qPCR results showed that the transcription of P16 and P21 were both up-regulated, but not that of P27. The protein levels of P16 and P21 were also increased by wogonin. Flow cytometry analysis confirmed that wogonin upregulated ROS level in MDA-MB-231 cells. Immuno-fluorescent staining and western blots showed that wogonin increased γH2AX levels in MDA-MB-231 cells. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
TXNRD2 |
| Target gene experiment: |
RT-qPCR//Western blot//Ch-IP-qPCR |
| Target gene description: |
RT-qPCR assay showed that wogonin downregulated mRNA level of TXNRD2 significantly. Protein abundance of TXNRD2 and H3K9Ac was measured by western blot. Reduction of H3K9Ac at the regulatory region of TXNRD2 after wogonin treatment was shown by ChIP-qPCR. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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