External factors
| External factors: |
ABT-263 |
| Category: |
Chemical compounds |
| Cell name: |
SK-BR-7,U2OS,A549,HCC712,MDA-MB-175,MCF-7 |
| Experiment: |
Western blot//Immunoblotting//Immunofluorescence//Microscopy//Gene editing |
| Description: |
Treatment with appropriate senolytic drugs induced strong activation of caspase 3, as detected by immunofluorescence staining and immunoblot, but only in senescent cells.Time course microscopy confirmed doxorubicin-induced senescent 4226 and MDA-MB-175 cells underwent rapid and near-synchronous cell death when treated with ABT-263 or ABT-263/S63845. To verify the role of BCL-XL and MCL1 in survival of senescent MCF-7 A/S-sensitive cells, we infected with CRISPR/Cas9 lentivirus and guide RNAs targeting either BCL2L1 (BCL-XL) or MCL1, and hypothesized edited cells would die when treated with only the MCL1 inhibitor S63845 or ABT-263, respectively. This hypothesis was supported as the number of senescent MCF-7 BCL2L1-sg cells was significantly reduced with S63845 alone, and senescent MCF-7 MCL1-sg cell number was reduced by treatment with ABT-263 alone. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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