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Aging Overview

External factors

External factors: 5-AZA-Dc
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Aging
Experimental category: HL
Tissue type: --
Cell name: Breast cancer cells,ovarian cancer cells
PMID: 33579350
Experiment: Western blot//Flow cytometry
Description: 5-AZA-dC treat- ment induced a significant increase in p21 expression in all cell lines compared to untreated controls. For Rb, there was a significant decrease in expression in PEO1 and PEO4 cells compared to untreated controls.The G1 phase was decreased for all 6 cell lines, reaching statistical significance for PEO1 and MDA-MB-231 treated cells. The S phase was significantly increased in PEO1 and MDA-MB-231 treated cells, and G2 phase was significantly increased in A2780cis, PEO1 and MDA-MB-231 treated cells (p?≤?0.05).



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Regulatory relationship

Target gene: MGAT5//ST3GAL4
R-EF-Target gene: Upregulation//Upregulation
Official symbol(s): MGAT5//ST3GAL4
Target gene experiment: RT-qPCR
Target gene description: Consistent with increases in highly branched and sialylated glycans, the expression of MGAT5 and ST4GAL3, glycosyltransferases responsible for branching and sialylation, was also increased in chemosensitive cell lines A2780 and PEO1 post-5-AZA-dC treatment.


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Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.




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