External factors
| External factors: |
High glucose |
| Category: |
Chemical compounds |
| Phenotype: |
Osteoarthritis |
| Experiment: |
Western blot//SA-β-gal activity assay |
| Description: |
The expression of senescence-related markers (p53, p21, and p16) increased markedly with increase in glucose concentration. SA-β-gal staining results revealed that HG significantly increased the number of SA-β-gal- positive cells in a concentration-dependent manner. |
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Regulatory relationship
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
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AMPK
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Western blot results revealed that the ratio of p-AMPK/AMPK decreased in a concentration-dependent manner. Western blot analyses results revealed that HG decreased the phosphorylation level of AMPK, whereas AICAR increased the phosphor- ylation level of AMPK, suggesting that AICAR has the capacity to activate AMPK in HG induced chondrocytes. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.