External factors
| Category: |
Chemical compounds |
| Experiment: |
Western blot/SA-β-gal activity assay |
| Description: |
In control siRNA-transfected HUVEC cells, H2O2 induces a remarkable increase in the phospho- ryaltion of Rb and 17b-E 2 significantly inhibits H2O2 s effect.17bE2 significantly inhibits H2O2-induced increase in the number of SA-b-gal-positive cells.(孟丽加的)17b-E 2 loses the capacity to thwart H2O2 -induced increase in the number of SA-b-gal- positive cells. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
SIRT3 |
| Target gene experiment: |
RT-PCR//Western blot//Luciferase reporter assay |
| Target gene description: |
As shown in the results from the real-time PCR, 17b-E 2 increases the expres- sion levels of SIRT3 mRNA in a dose-dependent manner in HUVEC cells.Immunot blot analysis shows that the protein expression levels of SIRT3 are elevated, when HUVEC cells are exposed to different concentrations of 17b-E 2. We con- structed a Luciferase-based reporter system to mea- sure the transcriptional activity of SIRT3. As expected, 17b-E 2 increases SIRT3 promoter activity and exhibits the maximum effect at the concentration of 0.1 μM. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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