External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Western blot//RT-PCR |
| Description: |
Exposure of WI-38 cells to H2O2 for 4 days significantly increased the number of SA-b-Gal-positive cells compared to the control group. However, treatment with CORM-A1 markedly reversed the number of H2O2-induced SA-b-Gal-positive cells. CORM-A1 significantly reduced the expression of TNF-a IL-6, p21, and PAI-1 in H2O2- induced senescent cells. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
MIR-34A |
| Target gene experiment: |
RT-qPCR |
| Target gene description: |
CORM-A1 strongly decreased the expression of miR-34a. |
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| Regulatory pathway:
|
miR-34a-Sirt1 |
| R-EF-Pathway:
|
Downregulation |
| Pathway experiment: |
SA-β-gal activity assay//RT-PCR |
| Pathway description: |
CORM-A1 treatment significantly increased both mRNA and protein of Sirt1 levels.CORM-A1 strongly decreased the expression of miR-34a. CORM-A1 significantly decreased the number of miR-34a-overexpressing SA-b-Gal-positive cells. Notably, p21 and PAI-1 were upregulated by miR-34a overexpression, whereas CORM-A1 significantly reduced miR-34a-induced p21 and PAI-1 levels. Furthermore, the miR-34a-induced Sirt1 downregulation was reversed by CORM-A1. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Pathway view
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