External factors
| External factors: |
Radiation |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Western blot |
| Description: |
On the other hand, 2 Gy of radiation induced senescence in both undifferentiated NT2/D1 and NT2/D1 1W cells in early phase of differentiation.Results also indicate that, after 2 Gy of radiation, both undifferentiated NT2/D1 cells and differentiated NT2/D1 1W cells have increased number of cells in sub-G1 phase, while number of cells in G1 and S phase is reduced .Also, we observed overall increased cell number in G0/G1 phase of NT2/D1 1W, accompanied with smaller number of cells in S phase compared to undifferentiated NT2/D1 cells .Western blot analysis showed that 2 Gy dose of radiation increased the level of p21Waf1/Cip expression only in NT2/D11W cells. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
471
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
472
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 751
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 754
Warning: Undefined variable $pathway_id in /data/html/Aging-ReG/public/browse_detail_2.php on line 793