External factors
| External factors: |
Hyperbaric air |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
Both HA- and NMH-treated cells exhibited the typical senescent cell morphology, such as cell enlargement, a reduced cytoplasm-to-nuclear ratio, saturation density, and increased sa-β-gal activity, after 7 days of culture . After this time point, the cells gradually displayed the characteristics of replicatively senescent cells in a time-dependent manner. About 50% blue-stained cells have been shown after 14 days of culture under both conditions. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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