External factors
| External factors: |
CD9-Lac/CaCO3/Rapa |
| Experiment: |
SA-β-gal activity assay |
| Description: |
HDFs were treated with free Rapa or CD9-Lac/CaCO3/Rapa for 72 h, followed by 3 passages before analysis because the proliferative effect of Rapa was found to be high after 3 passages.The number of HDFs (P23) positive for β-galacto sidase staining clearly decreased following treatment with Rap-a or CD9-Lac/CaCO3/Rapa, which can be attributed to mTOR inhibitory activities of Rapa.The population doubling time was signficantly reduced in free Rapa- or CD9-Lac/CaCO3/Rapa-treated HDFs (P23) when compared to untreated old HDFs. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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