External factors
| External factors: |
Radiation |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
Cell cycle analysis revealed induction of a G2 arrest in the total population of T47D cells after 5 × 3 Gy, from 14.7% to 21.5%. After fractionated radiation with 5 × 3 Gy, the total population of T47D cells showed a 3.6-fold increase of cells in the G0 phase of the cell cycle, from 3.31% to 11.9% .At 0 Gy, 2% of the total population was X-gal-positive/senescent cells . Fractionated radiation induced a dose dependent increase of X-Gal+/senescent cells in the total population, with a maximal effect at 5 × 3 Gy (25-fold increase, P = 0.09). |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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