External factors
| External factors: |
Nano-hydroxyapatite/chitosan/poly lactide-co-glycolide |
| Experiment: |
qRT-PCR//Western blot |
| Description: |
We detected markers of senescence in P3 or P27 hUCMSCs that cultured on conventional culture dish (hUCMSCs/CD) or nHA/CS/PLGA nano-scaffold (hUCMSCs/NS). As expected, the mRNA and protein levels of P53 were lower in P27 hUCMSCs/NS, as compared to hUCMSCs/CD. nHA/CS/PLGA scaffold prevented the increase of P53 proteins in hUCMSCs during in vitro passage.Culture on the nano-scaffold resulted in significant increases in the expression of BMP2, bFGF, EGF and CXCL5 in hUCMSCs when comparing with conventional culture dish . The expression of these above genes in P27 hUCMSCs/NS was ~10 times higher than that in P27 hUCMSCs/CD. nHA/CS/PLGA scaffold largely prevented the decline of these genes controlling stem cell function after culture of 27 passages. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
471
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
472
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 751
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 754
Warning: Undefined variable $pathway_id in /data/html/Aging-ReG/public/browse_detail_2.php on line 793