External factors
| External factors: |
Homologous recombination |
| Phenotype: |
Esophageal cancer |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Knockdown//Western blot |
| Description: |
To study the impact of HR suppression on the efficacy of telomerase inhibitor, HR in OE33 cells was first inhibited by lentivirus-based shRNAs targeting RAD51. RAD51-knockdown was confirmed by western blotting and HR suppression by plasmidbased assay. Cell growth: relative to control (CS) cells, the viability of RAD51-suppressed (R), GRN163L-treated CS and GRN163L-treated R cells was reduced by 34±5%, 24±6% and 65±5%, respectively. Thus,inhibition of HR or telomerase significantly reduced proliferation rate in OE33 cells, and combined treatment was associated with a significant 41% increase in cell death, relative to GRN163L used alone.Thus, simultaneous suppression of telomerase and HR led to a significant 36% (12-fold) increase in apoptosis, relative to telomerase inhibition alone.Evidence of senescence: cells were also evaluated for bgalactosidase staining, a marker for senescence. A subset of cells subjected to both telomerase and HR suppression stained positive for β-galactosidase, indicating senescence. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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