External factors
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis//Western blot |
| Description: |
Our data indicated that HPAEC underwent cellular senescence upon exposure to 10 Gy X-rays as determined by increased SA-β-gal activity, detected cytochemically as blue perinuclear staining at 4 days post-irradiation.X-ray-exposed HPAEC also exhibited changes in cell morphology, displaying unusually large cells and flattened cytoplasmic appearance compared to sham-irradiated controls. We also observed the upregulation of p21/waf1 within 3h post-irradiation, consistent with inhibition of the cell cycle in advance of senescence. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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