External factors
| External factors: |
Gamma irradiation |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
Then, the percentage of SA-β Gal stained cells was determined at different time points over 5 days after irradiation treatment. The highest percentage of SA-β Gal stained MRC-5 fibroblast cells (63 ± 4 %) was noted after the highest irradiation dose (20 Gy) and the longest time lapse (120 h) [72]. Therefore, HFF strains were only irradiated by 20 Gy. After 120 h the percentage of SA-β Gal stained HFF cells was similar to the corresponding value for the MRC-5 fibroblasts. protein expression levels of p21 and p16 were found to be significantly up-regulated in irradiation induced senescent fibroblasts compared to controls. Unexpectedly, a number of cytokines and cytokine receptors (IL11, EGFR, CXCL-1,2,3,5,6,14) were significantly down-regulated on irradiation induced senescence in MRC-5 and HFF strains, resulting in a significant down-regulation of the KEGG pathway “cytokine–cytokine receptor interaction” (hsa04060) representing SASP. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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