External factors
| External factors: |
NES-hTERT |
| Experiment: |
Growth curve assay//SA-β-gal activity assay//Flow cytometry//Cell cycle analysis |
| Description: |
NHF-expressing NES-hTERT arrested growth prematurely,Growth arrest was accompanied by flattened and enlarged morphology reminiscent of senescent cells,DNA profiles using propidium iodide (PI) and flow cytometry indicated that the cells were arrested in the G1?S transition, which is typical of the growth arrest associated with senescence. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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