External factors
| External factors: |
Chronic hypoxic conditioning |
| Experiment: |
SA-β-gal activity assay//ELISA |
| Description: |
SA-β-gal activity assay:An increase in blue staining in the hypoxic population indicates a notable presence of senescent-associated b-galactosidase activity.ELISA: the protein levels of key cytokines interleukin-1b (IL-1b), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor (TNFa) were determined in the media of the sublines using a multiplex ELISA system. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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