External factors
| External factors: |
Convallatoxin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Cell morphological analysis//Western blot |
| Description: |
Exposure of A549 cells to CON at 10 and 100 nM showed a significant increase in cells in the G2/M phase at all times tested, followed by an increase in cells in subG1 over the time.We also evaluated the expression of a key molecular driver of G2/M phase, cyclin B1, after 24 h of treatment by WB analysis.CON down-regulates cyclin-B1. CON induced at 50 nM an increase of ~80% of β-gal-positive cells. At 10 nM, an increase of larger regular nuclei was observed as well as the cells become flat, increase their volume and display a vacuole-rich cytoplasm, characteristic of senescence. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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