External factors
| External factors: |
Valproic acid |
| Category: |
Chemical compounds |
| Phenotype: |
Prostate cancer |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: |
Contrary to DU-145 cells, in PC-3 cells, a 24 h-exposure to 2 mM VPA caused a G2 cell cycle arrest.The light scatter plot shows enlargement and flattening of cells with increased granularity in the VPA-treated group.Induction of senescence (blue β-gal staining) and inhibition of proliferation (brown Ki-67 staining)simultaneously is depicted .By examining SA-β-gal activity we confirmed that VPA did induce cellular senescence in PCa. The greatest induction of senescence was in DU-145 cells exposed to 0.4% w/v VPA. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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