External factors
| External factors: |
LY2801653 |
| Category: |
Chemical compounds |
| Phenotype: |
Cholangiocarcinoma |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
We have analyzed senescence by measuring the senescence associated β-galactosidase (SA-β-Gal) activity assay in TFK-1 and SZ-1 cells after treatment with DMSO and LY2801653. In both cell lines we observed a significant increase in the percentage of SA-β-Gal activity after 48 and 96h compared to their controls.Moreover P21 is one of the major players linking cell cycle arrest and induction of senescence . We observed a strong expression of P21 compared to the untreated controls under 10mM LY2801653 at 96h. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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