External factors
| External factors: |
MLN4924 |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: |
We performed a β-galactosidase staining assay and found that most MLN4924-treated OS cells were stained , confirming MLN4924-induced senescence, In flow cytometry cell cycle analysis using propidium iodide DNA staining, a low concentration of MLN4924 (0.04 uM) modestly affected the cell cycle distribution, and a high concentration (1 μM) increased the population of cells in the G2 phase while reducing the S phase population.Furthermore, we observed that a fraction of cells became flat and greatly enlarged after MLN4924 treatment, suggesting an increase in cell senescence. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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