External factors
| Category: |
Chemical compounds |
| Phenotype: |
Urothelial carcinoma |
| Cell name: |
HEK293,UM-UC-3,VM-CUB1,HBLAK |
| Experiment: |
Flow cytometry//MTT assay//SA-β-gal activity assay//Cell morphological analysis |
| Description: |
Based on the results from the MTT assays, in the following experiments, cells were usually treated with 2 μM 19i or with 2.5 μM SAHA. Treatment with 2 μM 19i impaired the clonogenic potential of UC cells comparably to treatment with the pan-HDACi SAHA. This was also the case for HEK-293 cells, but HBLAK again were less sensitive .Efficacious concentrations of 19i elicited an increased fraction of cells in the G2/M phase in UC cells and in the non-urothelial HEK-293 cells.Over time, cells became larger and flatter, indicative of cellular senescence .Indeed, some 19i-treated cells, especially from the VM-CUB1 cell line, stained positive for senescence-associated β-galactosidase. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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