External factors
| External factors: |
Rapamycin |
| Category: |
Chemical compounds |
| Experiment: |
MTT assay//SA-β-gal activity assay//Western blot |
| Description: |
After treatment with rapamycin, proliferation of all senescent cells was dose-dependent, with the highest cell viability observed at 25 nm rapamycin. When cells were treated with rapamycin,a reduction in SA-β-gal-positive cells was observed(19.39?±?2.42%).Western blot analysis showed a significant decrease in the levels of p53 and p21 after treatment with rapamycin. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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