External factors
| External factors: |
Furanoflavones pongapin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Western blot |
| Description: |
ANF's EC50 value for inhibition of MCF7-1A1 cell proliferation was determined to be > 20 μM. Nevertheless, the methanolic extract (EC50 for inhibition of proliferation of MCF7-1A1, 3.8 ± 0.4 μg/mL) from the seeds of P. pinnata, and the plant's secondary metabolites pongapin (EC50 for inhibition of MCF7-1A1 proliferation, 0.6 ± 0.15 μM), lanceolatin B (EC50 for inhibition of MCF7-1A1 proliferation, 0.7 ± 0.12 μM) do block MCF71A1 cells at G0-G1 and repress cyclin D1 levels , when cells were treated with 2× EC50 concentration of extract or metabolites.Interestingly, it was found that the P. pinnata seed-extract, pongapin and lanceolatin B do induce β-galactosidase in MCF7-1A1 cells. Approximately 60–70% cells were positive for β-galactosidase activity out of which more than 20% cells were strongly positive (cells staining dark blue). The control MCF7-1A1 cultures showed less than 10% cells positive for β-galactosidase and were weakly stained (cells stained light blue). |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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