External factors
| Category: |
Chemical compounds |
| Experiment: |
Cell morphological analysis//SA-β-gal activity assay//qRT-PCR//Western blot//DAPI staining//Flow cytometry |
| Description: |
SA-β-gal activity assay: H2O2-treated NIH3T3 cells gradually became enlarged, flattened and were largely positive for SA-GLB1 staining from day 3. DAPI:The SAHFs in these cells were evident as large and irregularly shaped nuclear puncta. The arrest of cell growth was apparent,Western blot:and the levels of TRP53 protein. qRT-PCR:and Cdkn1a, and Il6 mRNA were elevated. Flow cytometry:H2O2 treatment also elevated intracellular ROS . |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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