External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
After treatment with hydrogen peroxide, the level of intracellular ROS was measured using H2DCFDA, which is converted to a fluorescent product, DCF, upon oxidation [37]. The ROS accumulation after hydrogen peroxide treatment in hUCB-MSCs was greater than that in human fibroblast and cancer cells.While the morphologies of the human fibroblast cells did not change significantly, hUCB-MSCs became larger and flatter after treatment with hydrogen peroxide. Staining cells with SA-β-Gal, a marker for detecting cellular senescence, revealed that hUCB-MSCs underwent senescence. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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