External factors
| External factors: |
Genistein |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
The effect of genistein on SA-β-gal activity was also observed and genistein was found to effectively suppress the expression of SA-β-gal in a dose-dependent manner.It was observed that treatment of HDFs with 40 and 80 mg/ml of genistein for 24 h decreased the number of early apoptotic cells (LR) significantly when compared to 18.1% in vehicle control (0 mg/ml) group.HDFs in G0/G1 phase was diminished in quantity upon different doses of genistein (20 mg/ml, 40 mg/ml, and 80 mg/ml), indicating a dose-dependent inhibition in cell cycle arrest by genistein. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Target gene:
|
P66SHC//FKHRL1 |
| R-EF-Target gene:
|
Downregulation//Downregulation |
| Official symbol(s): |
P66SHC//FOXO3 |
| Target gene experiment: |
Western blot//qRT-PCR |
| Target gene description: |
p66Shc and FKHRL1 were significantly up-regulated by UVB irradiation, and genisteintreatment of HDFs result edinadose-dependent decrease in their expression. The phosphory-lation of p66Shc and FKHRL1 were also down-regulated by different concentrations of genistein, which indicated genistein, as one of antioxidants, counteracts the UVB-induced oxidative stress at least in part by regulating the expression and activation of p66Shc and FKHRL1 protein. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
471
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
472
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 751
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 754
Warning: Undefined variable $pathway_id in /data/html/Aging-ReG/public/browse_detail_2.php on line 793