External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Immunofluorescence staining//SAHF |
| Description: |
TMZ is able to induce senescence in LN-229 and, to a lesser extent, in U87 MG cells. Senescence starts to become detectable 48 and 72 h after TMZ addition in LN-229 and U87 MG cells, respectively, and persisted over the whole post-incubation time period (up to 144 h). We also performed a cytochemical senescence detection assay using the chromogenic substrate 5-bromo-4-chloro-3-indoyl β-D-galactopyranoside (X-gal), which yields an insoluble blue compound when cleaved by β-gal. Further, we used another marker for senescence, the senescence-associated heterochromatic foci (SAHF), which are usually enriched for histone H3 methylated on lysine 9 (H3K9), which were not observed in non-treated but induced in TMZ-treated cells. |
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Regulatory relationship
| Target gene experiment: |
Flow cytometry |
| Target gene description: |
The data shows that 3-MA completely abolished senescence after TMZ treatment. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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