External factors
| External factors: |
Doxorubicin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//BrdU assay//Cell morphological analysis |
| Description: |
Doxorubicin induced a flat, senescence-like cell morphology, increased senescence-associated β-galactosidase (SA-β-gal)-positive cells to approximately 80%, and blunted BrdU incorporation into the DNA of proliferating cells by > 95% ;IFN-γ + TNF also induced the flat senescence-associated morphology,increased SA-β-gal activity. |
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Regulatory relationship
| Target gene experiment: |
Western blot//Immunostaining//qPCR |
| Target gene description: |
Ago2 translocation could also be demonstrated by western blot analysis of nuclear extracts of doxorubicin-treated MCF-7 cells；Cytokine-induced translocation of Ago2 into the nucleus of MCF-7 cells was verified by western blot analysis of nuclear extracts. A204 rhabdomyosarcoma (blue dots) or MCF-7 breast cancer cells (red dots) confirmed the downregulation of all five cell cycle genes which are known to be regulated by Ago2. after 24 h and 48 h. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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