External factors
| External factors: |
Ceramide |
| Category: |
Chemical compounds |
| Experiment: |
Cell morphological analysis |
| Description: |
Young HDF were able to label 67.5 ± 9.5% of their nuclei by [3H]thymidine in response to serum stimulation. In contrast, senescent cells had a labeling index of <2% , cells grown in 10 μM C6-ceramide had a labeling index of 12 ± 5% ,and cells grown in 15 μM C6-ceramide had a labeling index of 2.5 ± 0.5% and morphologically resembled senescent cells. |
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Regulatory relationship
| Official symbol(s): |
JUN//RB |
| Target gene experiment: |
Western blot |
| Target gene description: |
Upon serum stimulation young HDF are able to respond by activation of AP-1 (lanes 1 and 2), whereas ceramide-treated cells (lanes 5 and 6) like senescent cells (lanes 7 and 8) were unable to respond by activation of AP-1. Remarkably, C6-ceramide in concentrations ranging from 3 to 20 μM was able to progressively inhibit Rb phosphorylation in young HDF in response to serum stimulation as indicated by Western blot analysis . In addition, C6-ceramide (15 μM) was able to induce complete Rb dephosphorylation by 20-24 h, thus mimicking senescent HDF. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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