External factors
| External factors: |
Nitric oxide |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//qRT-PCR //ELISA |
| Description: |
NO treated HeLa cells stained positive for SA-βgal activity, a hallmark of cellular senescence and the percentage of stained cells increased proportionally to the dose of SNP . The senescent state was further confirmed by recording the induction in the expression of p21, NF-κb and IL8 and reduction in the expression of proliferation cell nuclear antigen (PCNA)at RNA level. Additionally, the levels of IL8 and IL6 cytokines, a part of the senescence associated inflammatory phenotype were also significantly elevated after SNP or DETA. |
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Regulatory relationship
| R-EF-Target gene:
|
Activation |
| Target gene experiment: |
Cell viability assay |
| Target gene description: |
Towards this, cells were treated with NO in the presence of a specific ATM kinase inhibitor, KU-55933 [46]. It is known that ATM kinase inhibition leads to an increase in ROS levels [44,45,47], which was observed when HeLa cells were treated with KU-55933, which increased further on NO treatment and was accompanied with significant loss of cell viability, as determined by a reduction in their proliferation as well as their metabolic activity . |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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