External factors
| Category: |
Chemical compounds |
| Experiment: |
Cell morphological analysis//SA-β-gal activity assay//Flow cytometry |
| Description: |
Cells were treated with C-1311 and stained for SA-β-gal, a marker of senescence. In A549 cells, increased SAβ-gal staining was visible within 48 hours of treatment. After 8 days of continuous drug exposure, more than 80% of the cells displayed SA-β-gal activity and flattened and enlarged morphology that was consistent with senescence phenotype. Although H460 cells also stained positively for SA-β-gal, the percentage of cells undergoing senescence was 2-fold lower than in A549 cells.In contrast, C-1311 exposure led to the substantial accumulation in the G1 phase in H460 cells,although G2-M arrest was also evident. |
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Regulatory relationship
| Target gene:
|
BECLIN1//ATG5 |
| Official symbol(s): |
BECLIN1//ATG5 |
| Target gene experiment: |
-- |
| Target gene description: |
SAβ-gal staining indicated that knockdown of Beclin 1 significantly attenuated C-1311–induced senescence in A549 cells.After 144 hours of C-1311 exposure, downregulation of ATG5 in A549 cells resulted in an approximately 25% inhibition of senescence compared with ATG5-positive cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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