External factors
| Tissue type: |
Adipose,Bone marrow,Dental pulp,Perinatal tissue |
| Experiment: |
SA-β-gal activity assay//qRT-PCR |
| Description: |
Cells(culture passages 5th–7th) were treated for 72 h with ZF1 at the final concentrations of 0.01,10,and 20 μg/mL. Although 0.01 μg/mL ZF1 was ineffective,both 10 and 20 μg/mL ZF1 significantly reduced the number of senescent hASCs positively blue stained for SA β-gal (p < 0.05).In contrast, hASCs exposed to both 10 μg/mL and 20 μg/mL ZF1 resulted in a similar statistically significant increase in TERT transcription as compared with the control hASCs (SOLV). |
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Regulatory relationship
| Target gene:
|
BMI1//P53//P21 |
| R-EF-Target gene:
|
Upregulation//Downregulation//Downregulation |
| Official symbol(s): |
BMI1//P53//P21 |
| Target gene experiment: |
qRT-PCR |
| Target gene description: |
We show that,in hASCs,ZF1 was also able to enhance the gene expression of BMI1, a pleiotropic transcriptional regulatoracting as a major repressor of senescence .On the contrary,in hBM-MSCs,hDP-MSCs,and hWJ-MSCs, he ZF-induced increase in TERT transcription was associated with a down-regulation in both TP53 (p53) and CDKN1A(p21) transcription. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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