External factors
| External factors: |
Malignant ascitic fluids |
| Phenotype: |
Ovarian cancer |
| Cell name: |
HPMC,A2780,SKOV3 |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
By doing so, we found that the percentage of cells in the S-phase of the cell cycle in cultures exposed to malignant ascitic fluids was decreased by 40 ± 2 % (p < 0.01) compared to their counterparts subjected to the control ascitic fluids. This effect coincided with an increased activity of SA-β-Gal, an indicator of cellular senescence, by 25 ± 5 % (p < 0.03) compared to the control group .At the same time, we found that cultures exposed to benign ascitic fluids contained ~10 ± 4 % prematurely senescent cells (no significant difference), whereas in cultures exposed to malignant ascitic fluids the subset of senescent cells increased to34 ± 4 % (p < 0.01). |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation//Upregulation |
| Official symbol(s): |
CXCL1//HGF |
| Target gene experiment: |
SA-β-gal activity assay |
| Target gene description: |
We found that the concentrations of 3 of these factors, i.e. GRO-1, HGF and TGF-β1, differed in malignant versus benign ascitic fluids. The levels of these factors were uniformly higher in the malignant ascitic fluids.We found that the activity of SA-β-Gal in the HPMCs was increased when they were exposed for 72h to exogenous GRO-1. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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