External factors
| External factors: |
tris-C60 |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
Interestingly, unlike hexa-C60, all doses of tris-C60 induced a significant increase (～12%) in cells arrested in G0/G1phase with fewer cells entering the S phase.No senescent cells were observed in the control samples.However, all doses of tris-C60(from 25 to 100 μg/ml) induced significant senescence at 24 and 48 h. Moreover, when tris-C60was removed by washing and cells incubated with fresh medium, the number of cells undergoing senescence was significantly reduced 24 and 48 h post-tris-C60treatment, suggesting that tris-C60 induced senescence was reversible. |
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Regulatory relationship
| Target gene:
|
HERC5//P16//P21//P53 |
| R-EF-Target gene:
|
Downregulation//Upregulation//Upregulation//Upregulation |
| Official symbol(s): |
HERC5//P16//P21//P53 |
| Target gene experiment: |
RT-PCR//Western blot |
| Target gene description: |
A significant number of cell cycle related genes were altered by less than 2-fold in response to 100 μg/ml tris-C60 treatment at the 24 h time point. Surprisingly, a notable 5-fold decrease was observed in the expression level of HERC5, a cyclin E binding protein. These results were confirmed with Taqman RT-PCR using different concentrations of tris-C60.A significant and simultaneous decrease in the level of HERC5 protein was noted in the tris-C60treated cells, with no detectable levels at the 100 μg/ml dose.An increase in p16,p21 and total p53 protein expression levels was observed in the tris-C60 treated cells indicating the involvement of these key cell cycle regulatory proteins. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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