External factors
| External factors: |
Ionizing radiation |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
We confirmed that incidence of the senescence-like phenotype increased after exposure to 4-Gy IR. Like in senescent cells, cells began to exhibit an enlarged and fl attened cell shape and increased their SA- β -gal activity up to 22% and 39%, respectively, at 2 and 4 Gy of IR. |
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Regulatory relationship
| Official symbol(s): |
IGFBP5 |
| Target gene experiment: |
MTT assay//SA-β-gal activity assay//Tube formation assay |
| Target gene description: |
IGFBP5-downregulated cells were increased in cell proliferation compared with the control cells during irradiation. Based on SA- β -gal staining, IR increased SA- β -gal activity in control virus-transduced cells but not in IGFBP5 miRNA transduced cells. By tube formation assay, we observed that decrease in endothelial cell tube formation by irradiation was recovered by IGFBP5 down-regulation.These results demonstrate that IR-induced IGFBP5 is associated with radiation-enhanced cellular senescence and IGFBP5 downregulation can regulate radiation damage. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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