External factors
| Experiment: |
Growth curve assay//SA-β-gal activity assay |
| Description: |
The recorded cell counts demonstrated increased cell proliferation under 21% O2 compared to 5% O2with a major significant gap after 72 h.The percentage of blue cells is significantly higher in old fibroblasts grown under 21% O2 compared to those grown under 5% O2. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Target gene:
|
MIR-181A//MMP1//COL1A1 |
| R-EF-Target gene:
|
Downregulation//Upregulation//Downregulation |
| Official symbol(s): |
MIR-181A//MMP1//COL1A1 |
| Target gene experiment: |
qPCR |
| Target gene description: |
Our results show that in comparing cells cultured under 21% O2 with those cultured under 5% O2, we observe downregulation of miR-181a in young cells while an opposite highly significant upregulation was observed in old cells.The results show that there is a significant activation of MMP1 mRNA expression in both young and old cells, indicating that HDF cultured under 21% O2are more aged with respect to those cultured under 5% O2. The same statement is valid also for mRNA expression of COL1A1 which is significantly downregulated under 21% O2 in old cells compared to 5% O2, an indication that cells are more aged. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
471
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
472
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 751
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 754
Warning: Undefined variable $pathway_id in /data/html/Aging-ReG/public/browse_detail_2.php on line 793