External factors
| External factors: |
PTC-209 |
| Category: |
Chemical compounds |
| Cell name: |
MRC-5,MCF-7,MDA-MB-231 |
| Experiment: |
SA-β-gal activity assay//Western blot//EdU assay |
| Description: |
The stained cells were counted and plotted. We also examined the expression of senescence-associated proteins such as p21, p53, pRB and p16. The results showed that the PTC-209 strongly induced expression of p53, p21 and p16, and increased expression of hypo-phosphorylated pRB in MRC5 cells. Furthermore, PTC-209 strongly induced premature senescence in these cells as indicated by increase in SA-β-gal positive cells and corresponding decrease in EdU positive cells. PTC-209 also strongly induced premature senescence in MDA-MB-231 and MCF7 breast cancer cells. |
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Regulatory relationship
| Target gene:
|
MIR-200C//MIR-141 |
| R-EF-Target gene:
|
Upregulation//Upregulation |
| Official symbol(s): |
MIR-200C//MIR-141 |
| Target gene experiment: |
qRT-PCR//Luciferase reporter assay//EdU assay//Western blot//SA-β-gal activity assay |
| Target gene description: |
PTC-209 treatment resulted in upregulation of both miR-200c and miR-141 . The results of qRT-PCR were further confirmed by promoter-reporter assays, which showed that the PTC-209 upregulated miR-200c/141 promoter activity in a dose-dependent manner .The results showed that the PTC-209 strongly induced p21 in control IH cells and that inhibition of either miR-200c or miR-141 could suppress p21 induction by PTC-209. The results indicated that the PTC-209 treatment led to the induction of premature senescence in control IH cells but not in cells expressing inhibitors of either miR-200c or miR-141. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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