External factors
| External factors: |
Rapamycin |
| Category: |
Chemical compounds |
| Experiment: |
Cell morphological analysis//SA-β-gal activity assay//Flow cytometry |
| Description: |
Rapamycin was added to REF cells at passage 7 , and the cells were grown for 3 additional passages in rapamycin-containing media. Afterwards, rapamycin was removed, and during the subsequent passages the cells were becoming smaller in size,thereby reflecting a gradual restoration of the non-senescent phenotype as evidenced by Giemsa staining.The population lost such senescence markers as SA-β-Gal staining and restored the lost ability to proliferate as evidenced by an increase of S-phase cells. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
MTERT |
| Target gene experiment: |
RT-PCR |
| Target gene description: |
Interestingly, transcription of telomerase gene (mtert) is additionally augmented in rapamycin-derived cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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