External factors
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Cell name: |
Fibroblast,IMR-90 |
| Experiment: |
SA-β-gal activity assay//Western blot//BrdU assay |
| Description: |
Ectopic expression of GATA4 induced senescence in human foreskin fibroblasts and IMR-90 fibroblasts ,as shown by increased senescence-associated β-galactosidase (SA-β-Gal) activity and decreased 5-bromo-2′-deoxyuridine (BrdU) incorporation. More important, depletion of GATA4 with stably expressed short hairpin RNAs (shRNAs) partially decreased IR-induced SA-β-Gal activity and delayed replicative senescence. |
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Regulatory relationship
| R-EF-Target gene:
|
Activation |
| Official symbol(s): |
NFKB1 |
| Target gene experiment: |
Immunocytochemistry//Western blot |
| Target gene description: |
GATA4 expression triggered NF-κB activation, and GATA4 depletion inhibited NF-κB activation during senescence. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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