External factors
| External factors: |
Nucleus accumbens-1 |
| Cell name: |
SKOV3/N130,HeLa/N130 |
| Experiment: |
SA-β-gal activity assay//BrdU assay/Knockdown//Western blot//Flow cytometry |
| Description: |
We observed that during activation of this NAC1 deletion mutant, the cells showed a decreased proliferation rate upon continuous passaging as compared with the cells without activation of this NAC1-dominant negative mutant. We further found that the decreased proliferation was accompanied by increases in the numbers of SA-β-gal–positive cells and in the numbers of cells with altered cellular morphology such as flattening and enlargement.p21, a cyclin-dependent kinase inhibitor and an inducer of cellular senescence,was also increased. Inactivation of NAC1 also significantly enhanced the proliferation-inhibitory and the colony formation–inhibitory effects, and caused a G2–M cellcycle arrest, suggesting that senescence occurs in G2–M phase. |
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Regulatory relationship
| Target gene experiment: |
Knockdown//Western blot//SA-β-gal activity assay |
| Target gene description: |
We found that the expression of DNp63 protein was markedly downregulated in cells with deficiency of NAC1 after treatment with g-irradiation ,The role of DNp63 in NAC1-mediated evasion of senescence was further verified by overexpressing DNp63 in NAC1-deficient cells.Ectopic expression of DNp63 in SKOV3/N130 or HeLa/N130 cells with inactivation of NAC1 or in A2780 cells with silencing of NAC1 significantly blunted the activation of senescence caused by g-irradiation. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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