External factors
| External factors: |
Low dose Emodin |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
In order to confirm the synergistic effect of Emodin and 5-FU in cellular senescence, SA-β-gal staining was detected and the results showed that cellular senescence (shown as SA-β-galt blue cells) was obviously detectable in sequential Emodin and 5-FU treatment at low dose. Hence, we investigated changes in the expression of senescence marker,p21, p16, p27 and E2F1 protein. The western blot analysis confirmed the up-regulation of p21, p16, p27 protein and the downregulation of E2F1 protein after the sequential treatment at low dose . |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
NRARP |
| Target gene experiment: |
Western blot |
| Target gene description: |
The western blot analysis has confirmed the down-regulation of NRARP protein after the sequential treatment at low dose. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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