External factors
| External factors: |
Salicin |
| Category: |
Chemical compounds |
| Phenotype: |
Vascular disease |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
When endothelial cells were exposed to 10 ng/mL TNF-a, those not treated by salicin displayed significantly greater cellular senescence after 48 h, as compared to cells that were not exposed to TNF-a. In contrast, salicin prevented senescence in a dose-dependent manner; endothelial cells treated with 50 and 100 mM of salicin showed proportionately less cellular senescence, with the 100 mM assay relative SA-β-Gal level of the 100 mM assay approaching that of the assay not treated with TNF-a.Cell cycle analysis indicated that whereas TNF-a-affected cells showed a higher level of cell cycle dysfunction compared to cells that were not exposed to TNF-a, salicin demonstrated a protective effect against cell cycle disruption at G0/G1. |
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Regulatory relationship
| Target gene experiment: |
Western blot |
| Target gene description: |
While nuclear levels of NRF2 were reduced in the group exposed to TNF-a, replicating previous research that TNF-a hinders nuclear translocation of NRF2. Importantly, we found that salicin promoted nuclear translocation of NRF2. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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