External factors
| External factors: |
5-Aza-20-Deoxycytidine |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//SAHF//Immunostaining//Cytokine array |
| Description: |
Incubation with 5-aza-dC led to a significant increase (P < 0.001) in the number of SA-β-gal–positive HepG2 cells；We therefore investigated SAHF formation by immunofluorescence microscopy of H3K9me3 in HepG2 cells.Treatment with 20mmol/L 5-aza-dC for 72 hours triggered not only the typical increase of cell size, but also resulted in the characteristic accumulation of H3K9me3 in subnuclear dots.The incubation with 5-azadC caused increased levels of sICAM-1, interleukin (IL)1ra, and IL-8 in the culture supernatants. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation//Upregulation |
| Official symbol(s): |
P16//P53 |
| Target gene experiment: |
Immunohistochemistry//Western blot |
| Target gene description: |
In this experimental setting, only 5-aza-dC but not 5-aza-CR induced an upre-gulation of p16(INK4a)；In contrast, 5-aza-dC rather led to an increase in p53 protein levels, which might be related to the senescence-inducing capacity of this drug. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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