External factors
| External factors: |
Nicotinamide |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
When treating with nicotinamide alone, the percentage of SA-β-gal-positive cells increased to 12.3 ± 2.5%; after treating with H2O2, nicotinamide significantly counteracted DECM-mediated anti-senescent effects by raising the percentage of SA-β-gal-positive cells to 57.2 ± 5.5% . |
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Regulatory relationship
| Target gene:
|
P16//SIRT1//P38 |
| R-EF-Target gene:
|
Upregulation//Downregulation//-- |
| Official symbol(s): |
P16//SIRT1//P38 |
| Target gene experiment: |
Western blot//RT-PCR |
| Target gene description: |
The treatment with nicotinamide significantly increased p16INK4αin the H2O2-treated cells by 52.4% at the mRNA level and by 4.2-fold at the protein level compared with the untreated cells.The nicotinamide supplement also decreased SIRT1 in the H2O2-treated cells by 22.8% at the mRNA level and by 68.9% at the protein level .The western blot assay showed that exposure to nicotinamide and H2O2 increased phosphorylation of p38 by 4.2-fold compared with the control cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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