External factors
| External factors: |
Heat shock |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Cell morphological analysis//Western blot//DAPI staining |
| Description: |
Inhibition of proliferation was confirmed by cell staining with antibodies to Ki-67, a marker of proliferative activity. Seventy-two hours after heat shock, only a few cells were Ki-67-positive. Cell cycle analysis by flow cytometry revealed that heated cells were arrested mostly in G2/M phase.The modified morphology of MSCs subjected to HS: they are enlarged,more flattened, and express X-gal. Three days after HS, the number of SA-β-gal-positive cells increased to about 70 %. HS induced expression of p21 protein, a cell cycle inhibitor and major regulator of the senescence program. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Target gene experiment: |
Western blot//PCR |
| Target gene description: |
HS induced expression of p21 protein, a cell cycle inhibitor and major regulator of the senescence program. Four hours after recovery, p21 upregulation was obvious both at mRNA and protein levels. During 72 h after HS, the p21 expression gradually increases up to three times. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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