External factors
| Category: |
Chemical compounds |
| Experiment: |
Cell morphological analysis//SA-β-gal activity assay//Flow cytometry |
| Description: |
H2O2 treatment was found to lead to development of senescent-like morphology: cells become enlarged, flattened,and heterogeneous. senescent cells demonstrated SA-β-Gal staining which increased gradually,and the most remarkable effect was reached at 7 days after treatment: more 95% H2O2-treated cells were SA-β-Gal positive .The analysis of the cell cycle phase distribution in hMESCs showed that a pulse H2O2 treatment led to the arrest in all of the cycle phases . |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Target gene experiment: |
Flow cytometry//Western blot |
| Target gene description: |
200uM H2O2 promoted a significant elevation in protein expression of p21 in 7 h after treatment. it has been reported that, in bone marrow-derived mesenchymal stem cells exposed to sublethal doses of H2O2,a rapid decrease of proliferation rate was detected within 3 days and correlated with G1 phase arrest of the cell cycle when p21 was accumulated at the same time. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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