External factors
| Category: |
Chemical compounds |
| Phenotype: |
Prostate cancer |
| Experiment: |
Cell cycle analysis//qRT-PCR//ELISA//SA-β-gal activity assay |
| Description: |
After treatment with 0.5 μM RD, accumulation of cells with enlarged and flattened morphology was evident in PCa cells. The level of SASP mark IL-6 was found significantly increased in PCa cells after RD treatment by Elisa assay and q-PCR assay . In agreement with a large morphology, increased SA-β-gal was also observed in response to RD . The SA-β-gal positive stained cells was significantly enhanced by LNCaP cells,DU145 cells, respectively, as compared to controls. |
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Regulatory relationship
| Target gene experiment: |
SA-β-gal activity assay |
| Target gene description: |
Deletion of p21CIP1 by targeting siRNA impaired RD-induced senescent (SA-β-Gal positive) cells in all three PCa cells.SA-summ staining was decreased 0.32±0.05-fold in LNCaP, 0.43±0.19-fold in PC3, and 0.31±0.03-fold in DU145 cells, respectively, indicating that RD-induced cellular senescence was, at least in part, in a p21CIP1-dependent manner. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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