External factors
| External factors: |
MLN4924 |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Cell name: |
Lymphoma cell line |
| Experiment: |
PI staining//FACS analysis //Cell morphological analysis//SA-β-gal activity assay |
| Description: |
A prominent G2-M cell-cycle arrest was observed in all treated cell lines (Raji, U937,SU-DHL-4 and Toledo) in a dose-dependent manner；An increase in cell size with flattened shape, a characteristic of senescence, was observed in Raji and U937 cells;the expression of SA-β-gal was determined by SA-β-gal staining in MLN4924-treated cells，a substantial proportion of MLN4924-treated cells were positively stained. |
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Regulatory relationship
| Official symbol(s): |
P21//P27 |
| Target gene experiment: |
Western blot |
| Target gene description: |
MLN4924 significantly delayed p21/p27 turnover and extended the half-life of p21/p27 in both Raji and U937 cells.In contrast, MLN4924 had little effect on the transactivation of p21/p27 . |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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