External factors
| Category: |
Chemical compounds |
| Cell name: |
A375,G-361,SK-MEL-24 |
| Experiment: |
SA-β-gal activity assay//BrdU assay |
| Description: |
A time-dependent increase in β-gal staining was noted in cells treated with 10 nM LGX818 for periods of up to 72 h compared with vehicle-treated (non-treated) cells. Consistently, LGX818 treatment led to a significant decrease in the percentage of BrdU-positive cells. Elevated SA-β-gal activity and reduced BrdU incorporation were also observed in LGX818-treated SK-MEL-24 cells. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation//Activation |
| Official symbol(s): |
P27//PRB |
| Target gene experiment: |
Western blot |
| Target gene description: |
Immunoblot analysis demonstrated that A375 and G361 cells exhibited increased levels of p27KIP1 (CDK-inhibitory protein 1) protein 24 h after LGX818 treatment.We observed a remarkable decrease in the phosphorylation of Rb in A375 cells following a 24 h treatment with LGX818 and, to a lesser extent, in LGX818-treated G361 cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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