External factors
| External factors: |
Nutlin-3 |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
After a 4-d exposure to nutlin-3 at a dose (2 lM) that had hardly exerted any apoptosis-inducing effect, CADO-ES-1 cells were stained for SA-β-Gal activity, the most widely used marker for cellular senescence. Nutlin-3-treated cells showed clear signs of senescence, whereas untreated cells did not. |
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Regulatory relationship
| R-EF-Target gene:
|
Activation |
| Target gene experiment: |
Western blot//qRT-PCR |
| Target gene description: |
Immunoblot detection revealed a rise of p53 after nutlin-3 exposure in the ES cell lines with wt-p53.To confirm the p53-activating effect of nutlin-3, we determined the gene expression of three key transcriptional targets of p5326 MDM2, p21 and PUMA by real-time RT-PCR.Nutlin-3 induced concentration-dependent increases in mRNA levels in all three cases in the wt-p53 cell lines, but not in the mt-p53 one. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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